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Image Search Results
Journal: Frontiers in Immunology
Article Title: African swine fever virus MGF360-4L protein attenuates type I interferon response by suppressing the phosphorylation of IRF3
doi: 10.3389/fimmu.2024.1382675
Figure Lengend Snippet: MGF360-4L interacted and co-localized with IRF3. (A) MGF360-4L interacted with IRF3 in the overexpression system. HEK 293T cells were transfected with the indicated plasmids (5 µg each). IgG was used for control immunoprecipitation. Co-immunoprecipitation and immunoblotting analyses were performed with the indicated Abs. (B, C) Co-localization of MGF360-4L with IRF3. HEK 293T (B) cells and PK-15 cells (C) were transfected with MGF360-4L-Flag and IRF3-HA expression plasmids. After 24 h of transfection, MGF360-4L-Flag and IRF3-HA were stained with mouse-anti-Flag (green) and rabbit-anti-HA (red), and the nuclei were stained with DAPI (blue). Confocal assays were performed using Leica TCS SP8.
Article Snippet: The cells were harvested and washed with cold phosphate-buffered saline, then lysed on ice with the
Techniques: Over Expression, Transfection, Control, Immunoprecipitation, Western Blot, Expressing, Staining